Katya Heldwein
Harvard Medical School
Abstract:
We have recently solved the structure of
the clathrin adaptor protein complex, AP1, which crystallized in a very
long unit cell, a=b=180 angstrom, c=1100 angstrom, alpha=beta=90 deg,
gamma=120 deg. To record diffraction to 4.0 angstrom, while achieving
adequate spot separation, we have used the double 2X2 CCD detector setup
at CHESS F1 beamline. To minimize overlaps, the data were collected in
thin oscillation frames.
The structure was subsequently solved by molecular replacement method using a homologous clathrin adaptor protein complex, AP2, as a search model and data in a very low resolution range. The presence of NCS allowed us to calculate interpretable averaged maps, rebuild the structure and carry out refinement, which in turn, revealed atomic-level differences between the AP1 and AP2 structures.
2008 Run
Nov 19th - Dec 22nd
